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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312358426
中文名称:
英文名称:COMB, 2.0 MM, 12 SAMPLES, 1/CS, MA: FB57189
货期:热销品现货,其他请咨询




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文献和实验a syringe and needle, make sure all urea is rinsed out, load samples. Run gel at constant 40 mA for about 1 hr (BPB runs around 12nt and cyanol around 55nt.) UV-Shadow and Semi-dry transfer: Take out all buffer from top reservoir
with baculovirus, use a lysate from 3000 cells per lane in a 10 well comb with diluted sample buffer. # Heat samples at 95°C for 10 minutes before loading on the gel. Electrophoretic Separation of Proteins: For 1 gel, electrophorese initially at 20 mA
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
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