赛默飞世尔Thermo Fisher COMB, 2.0 M

小RNA的Northern BLOT

a syringe and needle, make sure all urea is rinsed out, load samples.  Run gel at constant 40 mA for about 1 hr (BPB runs around 12nt and cyanol around 55nt.) UV-Shadow and Semi-dry transfer:  Take out all buffer from top reservoir

美国实验室wetern方法

with baculovirus, use a lysate from 3000 cells per lane in a 10 well comb with diluted sample buffer. # Heat samples at 95°C for 10 minutes before loading on the gel. Electrophoretic Separation of Proteins: For 1 gel, electrophorese initially at 20 mA

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v