赛默飞世尔Thermo Fisher BIS(DI-TERT

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

in a yield of 40% after P (III) oxidation and purification. Complete removal of the nucleobases, phosphates, and 2??O ?tert ?butyldimethylsilyl protecting groups leads to the desired propargylated c?di?GMP diester. Cycloaddition of a biotinylated azide

v468 chapter 5 糖原合成酶激酶-3 的抑制

). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

; protocol 3 ) Tetrahydrofuran (THF), anhydrous, ≥99.9% pure tert ‐Butyl alcohol (t ‐BuOH), ACS grade, ≥99% pure