赛默飞世尔Thermo Fisher X12 Membran
e Vivaspin 20 5-20ml samples MWCO (PES) 100,000 Polyethersulfone (pack of 12) 货号：T_70310672422- ￥1200 - 24000
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- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310672422
中文名称：
英文名称：X12 Membrane Vivaspin 20 5-20ml samples MWCO (PES) 100,000 Polyethersulfone (pack of 12)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
. Sponge - filter paper - gel - membrane - filter paper - sponge 3. Transfer for 1 hr at 1 amp at 4°C on a stir plate. Bigger proteins might take longer to transfer. For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer
. Incubate tubes on ice for 15 min. 3. Clear cell lysate by centrifuging samples at 18,000× g in arefrigerated tabletop microcentrifuge for 15 min at 4°C. Transfer supernatant to a fresh microcentrifuge tube. 4. Measure proteins concentration
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