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文献和实验Clonality - X Chromosome Inactivation Assay
inactivation. Am J Hum Genet 51:1229-39, 1992 . Enomoto T, Fujita M, Inoue M, Tanzawa O, Nomura T, Shroyer K. Analysis of clonality by amplification of short tandem repeats. Carcinomas of the female reproductive tract. Diagnostic Molecular Pathology 3:292
We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
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