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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70101826E
中文名称：可加热塑料垃圾袋
英文名称：BG AUTO PP CLR 37X48 100/PK
货期：热销品现货，其他请咨询
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
) DMEM/F12 (Invitrogen, Catalog # 12500-096 or equivalent) Materials: BG01V human embryonic stem cells Tissue culture dishes (60 mm; Fisher, Catalog # 08-772B, 100 mm; Fisher, Catalog # 08-772E, or equivalent) 15 mL conical
【转贴】immunofluorescence staining
. (This is especially important for cells to be imaged at 100x with the deconvolution microscope; small structures may wiggle and result in blurry images, no kidding!).Remove fix (discard in toxic waste) and wash X 3 in PEM, 2-5 min per wash.To quench auto-fluorescence
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
9. After the last centrifugation, discard the supernatant and resuspend PBMCs at 2 x 106 cells/mL in warm (37°C) R10 medium. Aliquot a maximum 107 (or 5 mL) cells per 50-mL PP conical tube and incubate overnight in a humidified atmosphere incubator preset to 37°C
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