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999
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北京泽平
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EA
货号：T_70311827232
中文名称：
英文名称：X500 DISH HEXAGONAL 12 CM
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文献和实验RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
% confluence. For cells grown in suspension, skip to step 6. 2. Wash cells once with 2 to 3 ml per 10 cm dish of Trypsin/EDTA Solution. Aspirate solution immediately. 3. Add 2 ml of Trypsin/EDTA Solution per 10 cm dish. Incubate for 2 minutes at room
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