货号：353085 BD 细胞刮匙，18cm手柄1.8c

小鼠脑神经瘤细胞概述及培养方法！

培养基：MEM(货号:PM150410)＋10% FBS(货号:164210-500)＋1% P/S(货号:PB180120) 培养条件：气相：空气，95%；CO2，5% 温度：37℃ 细胞用途：仅供科研使用。 说明书下载：小鼠脑神经瘤细胞Neuro 2a 三、细胞特性 1）来源：脑神经母细胞瘤 2）形态：阿米巴样干细胞 3）含量：>1x106 4）污染：支原体、细菌、酵母和真菌检测为阴性 5）规格：T25 瓶或者 1mL 冻存管包装运输和保存：可选择干冰运输及发送复苏存活细胞方式：（1）干冰

The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro

at 4°C, and resuspend them in 4 mL of OP9 medium. 12. Transfer 1-mL aliquots of cells to four 10-cm dish each containing 9 mL of OP9 medium. Maintain the cells by splitting them at a ratio of 1-to-4, and passaging the cells

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

were made electrocompetent. After transformation with 3 μg of linear (NotI digested) hLhcgr -BAC cells were plated in LB-agar plates (diameter 25 cm) containing Stp/Cm and cTc, 15 μg/ml, and incubated overnight at 37°C. The number of colonies on the plate was low ( kb