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- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70107-201-774
中文名称:
英文名称:SINGLE BLOCK 48X0.2ML 1CS
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
of the 1000x tetracyclin stock solution . Inoculate with 25 to 50µl of the glycerol stock. Incubate in 37°C shaker incubator for 6-8 hours. Preparation of host cells from single colony Streak XL1-blue
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T
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