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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701R588900010F
中文名称:
英文名称:KCL COND STD 50,000 5S/CM
货期:热销品现货,其他请咨询




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文献和实验a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX
cllect frm mre than ne surce. Nte 2: Yu can prep each sample as yu receive it r wait until yu have a ~ 50g f tnsils. If yu decide t prep them right away then perfrm steps 2 t 4 and then freeze at -80℃. If yu want t wait until yu have ~50g f tnsil,yu
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
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