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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70315127915
中文名称:
英文名称:X750 Wipe Amplitude Sigma 457.2mm x 457.2mm (10 x bag of 75 wipes)
货期:热销品现货,其他请咨询




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文献和实验Lymphocyte Transformation 淋巴细胞转染
. Blood should be kept at room temperature prior to use in this procedure.Wipe the exterior of the tubes of blood with EtOH, divide evenly and transfer the blood into 2-50 ml tubes. Bring the volume of each tube up to 40 ml with wash mediaPlace 10 ml
GST fusion protein purification GST融合蛋白纯化
1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃2)extract cells (from 500ml)in 25 mls.Heintz Buffer plus triton (HBT)by gentle pipette
【转贴】immunofluorescence staining
of use, make a 10% stock solution of the Triton X-100 in PEM buffer and aliquot to microcentrifuge tubes covered with foil. Store the stocks at -20 C.Coverslips:Round coverslips will fit in 24 well trays, and can be obtained from:Fisher Scientific, Cat
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