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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
. A 50 ml culture typically yields enough RNA for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel
A quick RNA mini-prep for Neurospora mycelial cultures
for 200 gel lanes (see below), and, as noted, smaller culture volumes may be used. 2. Flat mycelial pads are easier to grind than mycelial balls. Therefore, filter cultures using a Buchner funnel onto Whatman #44 filter paper. Wrap flat mycelial pads
(below), prepare a humidified staining chamber (I typically use a 15cm Petri dish containing a round of Whatman filter paper with a square of Parafilm on top of that. Moisten the Whatman paper with water, drain the excess, and press the Parafilm flatly and evenly
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