赛默飞世尔Thermo Fisher VISCOMETER

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

FISH protocols for Drosophila

or PCR product, 2 m L DIG or biotin RNA labeling mix, 2 m L 10x transcription buffer, 1 m L RNAguard (40 U/m L), 2 m L RNA polymerase (20 U/m L), and RNAse-free water to a final volume of 20 m L. Incubate at 37°C for 2-4 h. For PCR templates

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

with intermediate forward and side-scatter. (Run T-cells as a size control). Viral Transduction: 1. Pre-coat 12 well plates with CH-296. To each well add 1.5 mL of Iscove's/20% FBS plus growth factors (SCF, IL-6, Flt-3L, IL-11). 2. Collect 5000 cells