赛默飞世尔Thermo Fisher VISCOMETER

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

重组DNA的分离、克隆与测序实验

1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989). 2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.  

重组DNA的分离、克隆与测序实验手册

Wang, Kala Iyer, Steve Toth, Goughay Zhang, Hua Qin Pan and other members of the Roe laboratory, both past and present.1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY