赛默飞世尔Thermo Fisher X100 Pipett

Immuno-Laser Capture Microdissection

(A) and immunostained for CD45RO (B). Immunostaining highlights T cells within the germinal center and in the perifollicular area and makes architectural features more evident. Magnification x100. Figs. C & D : Microdissection from germinal centers

Cajal Body Isolation Protocol

sucrose, 0.5 mM MgCl2 S3 solution: 0.5M sucrose, 25 mM Tris-HCl pH 9.0 SP1 buffer: 1M sucrose, 34.2% Percoll, 22.2 mM Tris-HCl pH 7.4, 1.11 mM MgCl2 SP2 buffer: 20% Percoll, 10mM Tris-HCl pH 7.4, 1% Triton X100, 0.5 mg/ml heparin HT buffer: 10

Stripping Western Blots

- Better than mRIPA for some protein-protein interactions but not as clean 1% NP40 50mM Tris pH 7.5 (Note: can increase to pH 8 to decrease background) 150mM NaCl 5mM EDTA Triton X100 Buffer #1 - Use for PAK-Nck