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文献和实验An Ultra-fast method of DNA extraction from Neurospora
the need for large numbers of flasks when growing many cultures for DNA isolation. 1. Inoculate plastic Petri plates containing 25 ml of 1X Vogels, 2 sucrose, 0.04 Tween-80 (to prevent conidiation) (Zalokar, M. 1954. Arch. Biochem. Biophys. 50:71-80
An Ultra-fast method of DNA extraction from Neurospora
when growing many cultures for DNA isolation. 1. Inoculate plastic Petri plates containing 25 ml of 1X Vogels, 2% sucrose, 0.04% Tween-80 (to prevent conidiation) (Zalokar, M. 1954. Arch. Biochem. Biophys. 50:71-80) plus any other required supplements
5 ml peripheral blood (anticoagulation by heparin) 1.5 ml PHA 1 cell culture flask, e.g. Falcon 250 ml prepare up to 10 flask (1 flask will yield about 50 slides) Steps: Incubate culture for 72 hours in CO2 cell culture incubator, mix
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