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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312357019
中文名称:
英文名称:X1000 VIAL INSERT FLAT BOTTOM 5 X 31MM POLYPROPYLE ne 250µL (pack of 1K)
货期:热销品现货,其他请咨询








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文献和实验so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
. Note: 300mA is the max on our power supply. 5’end-labeling of Probe: 1 μl 10x PNK buffer (fresh from NEB) 30 pmol probe DNA oligo 1.66 μl [γ -32P] ATP, 150 mCi/ml (= 250 μCi, 42 pmol) Vial
(from R. Plattner & A.M. Pendergast) 1. Following stimulation, place cells on ice and wash 2X with cold PBS 2. Lyse each 100 mm plate with 500-1000 μl of lysis buffer (50mM HEPES pH7.0, 150 mM NaCl, 10% glycerol, 1% triton-X-100, 1.5 mM MgCl
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