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货号：T_70311860000
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英文名称：PROBE TYPE K PENETRATION
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文献和实验and to the vacuum. Filter the lysate through the Whatman,changing it often as it gets clogged. Filtrate may now be stored a 4℃ until it is time to run over an affinity column. Note: Don't store for more than a couple of days at 4℃. References K.D.Puri,E.B
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, and resuspended in 300 µl of hybridization buffer. h. The probe is boiled for 1-2 hours. This step reduces the length of the probe for efficient penetration of embryos. e. Probe production is assayed using the following protocol. 1 µl of probe in hybridization
.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
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