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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311821345
中文名称：
英文名称：PROBE TYPE K PENETRATION
货期：热销品现货，其他请咨询
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文献和实验and to the vacuum. Filter the lysate through the Whatman,changing it often as it gets clogged. Filtrate may now be stored a 4℃ until it is time to run over an affinity column. Note: Don't store for more than a couple of days at 4℃. References K.D.Puri,E.B
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, and resuspended in 300 µl of hybridization buffer. h. The probe is boiled for 1-2 hours. This step reduces the length of the probe for efficient penetration of embryos. e. Probe production is assayed using the following protocol. 1 µl of probe in hybridization
.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
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