赛默飞世尔Thermo Fisher Comb60 samp

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

Stripping Western Blots

by tapping the tube (try to avoid getting beads stuck to the side of the tube, above the level of the reaction mixture). 14.) Incubate at 25oC (room temp.) for 25min, mixing occasionally. 15.) Add sample buffer to 1x (I usually add 20 μl of 3x

生物芯片实验手册（NIH）

of the labeled cDNA. 11. Take a 2-3 µl aliquot of the Cy5 labeled cDNA for analysis, leaving 18-28 µl for hybridization. 12. Run this probe on a 2% agarose gel (6cm wide x 8.5 cm long, 2 mm wide teeth) in Tris Acetate Electrophoresis Buffer (TAE