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文献和实验Phycoerythrin conjugation protocol
. I used 117 x 1 cm. You can't really get away with anything smaller. Use a long thin one rather than a short fat one, since the latter did not work for me. Separation of the conjugate from the free reagents is tricky. *
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
(differential interference contrast [DIC]; Leica; with thermo plate inserted at the objective stage Microscope (stereo dissecting; Leica) Oosight Imaging System (Cambridge Research & Instrumentation Inc.) Petri dishes (35-mm and 60-mm
(1.05 kg/cm2 ) on liquid cycle.) SOB agar plates containing 20 mM MgSO4 and the appropriate antibiotic Standard SOB contains 10 mM MgSO4 . SOB medium, for growth of culture to be transformed Prepare
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