赛默飞世尔Thermo Fisher ZOBELL S SO

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

cDNA Library Screening Protocol - ZAPII

at 30°C. 3) Spin for 10 min at 2000 rpm in a sterile 50 ml conical using Beckman GS 6R centrifuge. Carefully decant media and gently resuspend pellet in 15 ml of 10 mM MgSO4 (don't vortex). Dilute cells to OD600 = 0.5 with 10 mM MgSO

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v