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- T_70110-039G
- 2025年07月12日
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- 保修期：
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- 规格：
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货号：T_70110-039G
中文名称：
英文名称：FLASK ERLENM NM 300ML 12/PK
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文献和实验of each of the twelve dilutions and plate on nutrient agar plates to perform a colony count as in the preceeding section. 5. Set a spectrophotometer for 686 nm wavelength and be sure it is turned on and functioning properly. Adjust the dark current to 0% T. 6. Place a cuvette
, it is helpful to start several 25 ml cultures, each with a different dilution of the starter culture (e.g. 1:100, 1:300, 1:900). 2. Grow at 30 C shaking (250 rpm) until the OD600 nm ~ 1.0 (This is usually done overnight). *** When growing several strains
3. Transfer to a 50 ml conical tube and centrifuge for 10 min at 2000 x g at 4 C. 4. Resuspend each sample in 1 ml of 10 mM sodium azide and place on ice. 5. Calculate the volume of resuspended cells that would translate to an OD600 nm reading of 10
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