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999
- 供应商：
北京泽平
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- 保修期：
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货号：T_70110-200-24
中文名称：
英文名称：BEAKER BLUE SCALE 1000ML 6/CS
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文献和实验Small scale DNA preps for Neurospora crassa.
minutes (vortex briefly if the pellet doesn''t dissolve completely). 10. Add 200 microliters of chloroform:isoamyl alcohol (24:1), vortex briefly and spin for one minute. Collect the aqueous phase (approximately 220 microliters). This is a good stage
Yeast Nuclear Extract (Small Scale)
Inhibitors and DTT are added just before use (0.46g Benzamidine,0.69g DTT,15 ml PMSF,3 ml 500X Leupeptin,7.5 ml 200X pepstatin,0.6 ml 2,500X chymostatin). -------------------------------------------------------------------------------- Zymolyase 100T (ICN
Small scale DNA preps for Neurospora crassa.
to wick away the excess liquid. 9. Add 250 microliters of TE (may include RNAse here) to each pellet and incubate at 50 degrees C for 5-10 minutes (vortex briefly if the pellet doesn't dissolve completely). 10. Add 200 microliters of chloroform
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