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文献和实验Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J.Leatherwood,Ptashne lab) last modified Mon,Aug 27,2001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is added
Yeast Nuclear Extract (Small Scale)
mixing. -------------------------------------------------------------------------------- Alternative: Instead of recombinant lyticase,can also use Zymolyase 100T from ICN.Use 12-18 mg per prep.The amount required can vary from ~12-18 mg depending
?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder
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