赛默飞世尔Thermo Fisher X960 ART 20

超级感受态细胞的制备

a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

Gene Knockout Techniques(1) The Knockout Process

of sterile M9 buffer and letting the plate sit overnight. It helps to make the buffer up to 0.01% Triton-X-100 (100 ul in 1 liter), which doesn?t hurt the worms and keeps them from sticking to the plastic of tips and centrifuge tubes. In either procedure