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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_701686-16
中文名称:
英文名称:AMM PHOSPHATE TS/RS, 500 ML
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文献和实验Calcium Phosphate Transfection of Neurons in Primary Culture
after transfection wash cells 3X with transfection media (serumfree, add, aspirate), then add fresh transfection media (don't let cells dry out) volume of transfection media: well of 24-well plate: 500ul
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
ELISA PROTOCOL 【Yale University 】
1 hour at room temperature.4) Discard the primary antibody solution and wash 3X with TST.5) Add 50 µl of diluted secondary antibody conjugated to AlkalinePhosphatase. I use a 1:500 dilution of rabbit anti-mouse IgG
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