M8R,5@}EUCm&<4H35}4g~g1`{1GqsV

Single Primer ("Semi-Random") P

Reamplification 40 µL H2O 10 µL 10x M-PCRB 10 µL 4 dNTPs @ 2 mM each 10 µL Pn primer @ 5 µM 10 µL Po primer @ 0.2 µM 10 µL DNA from part 1 10 µL "T/TS" @ 0.4 u Taq/µL --------- 100 µL Load glass capillaries and amplify: 30"x94°C 30

利用植物蛋白质芯片研究蛋白磷酸化

a d .  S ci.  U SA   101,  9879-9884. 26.  Bradford, M . M . (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. A n a l.  B io c h e m .72,  248-254. 27. 

转基因麦类作物：未来前景

Assessment  of nematode  resistancein  wheat  transgenic  plants  expressing potato  proteinase  inhibitor  ( P I N 2 )   gene.  T ra n sg en .  R es.  1 4 ,  665-675. 24.  T o b ia s ，D.  J . ，M a n o h a ra n ，M . ，P ritsch ，C.  a n d  D a h leen ，L.  S