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999
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北京泽平
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EA
货号：T_70102-683-20
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英文名称：BD S-L 21G 12IN W/HOLDER 25/PK
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
solution, T solution, cell solution. 1) To make 1 mL of fibrin gel (3.3 mg/mL final fibrinogen concentration, 25 U/mL final thrombin concentration), create F solution in a conical tube, by adding 112 μl of the fibrinogen stock to 558 μl
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
conical centrifuge tubes (BD Biosciences, cat. no. 352097) Centrifuge 25‐cm2 (T‐25) tissue culture flasks (Thermo Scientific
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