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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701K562500-2440
中文名称:
英文名称:DRYING TUBE 24/40
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
【转贴】immunofluorescence staining
of use, make a 10% stock solution of the Triton X-100 in PEM buffer and aliquot to microcentrifuge tubes covered with foil. Store the stocks at -20 C.Coverslips:Round coverslips will fit in 24 well trays, and can be obtained from:Fisher Scientific, Cat
flour at -40oC until extraction. Add 20 ml preheated (to about 70oC by micro-oven) extraction buffer A and 50 ul proteinase K (20 mg/ml), and then stir gently using a spatula. Incubate at 55oC for 30 min. Add 20 ml chloroform : isoamylalcohol (24
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