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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.- Mounting media: 70% glycerol, 1mg/ml p
【转贴】immunofluorescence staining
m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
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