赛默飞世尔Thermo Fisher X2 FLASK,BA
FFLED,POLYCARBONATE FOR CELL GROWTH 2000ML (4110-2000) (PACK OF 2) 货号：T_70310094900- ￥70 - 1400
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北京泽平
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货号：T_70310094900
中文名称：
英文名称：X2 FLASK,BAFFLED,POLYCARBONATE FOR CELL GROWTH 2000ML (4110-2000) (PACK OF 2)
货期：热销品现货，其他请咨询
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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
and viability using Trypan Blue exclusion Method (see Method below) 2. Using cell density determined in step 1, calculate the split ratio needed to seed the new shaker flask at 3x105 cells/ml according to the desired final volume of shaker flask (3x105 cells
contrast, and that they are harvested in log or late log phase growth (between 3.5 and 9 x105 cells/ml). Thaw cells 7 days before scheduled fusion. Myeloma do not grow well after being in culture for more than a few weeks. Make sure
Fusion of Mouse, Rat, or Hamster Cell
-well plate containing 5 ml/well sterile PBS. Transfer the spleen to a Petri dish containing 50 ml sterile PBS. Prepare a single cell suspension by teasing the tissue with sterile forceps
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