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文献和实验Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
-818.2009.03142.x (2009). 6. Hutson, M. S., & Ma, X. Plasma and cavitation dynamics during pulsed laser microsurgery in vivo . Physical review letters. 99, 158104 (2007). 7. Venugopalan, V., Guerra, A., 3rd, Nahen, K., & Vogel
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