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北京泽平
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货号：T_70311754558
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英文名称：X25 RUBBER CAP 5 HOLES D34X41 H35
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
(original reference: "tissue culture made easy" by Christian LaMantia from the Magnuson lab) 1) Plate cells onto gelatinized plates (35 or 60 mm) without feeders and culture 24 hours. A 1:4 split is best for most lines. 2) Culture
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