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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
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- 保修期:
1年
- 规格:
EA
货号:T_701ACCU0833500
中文名称:
英文名称:SOD SULFATE 1M, FILLSOLN 500ML
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文献和实验Southern Hybridization Protocols
℃.Prehybridize at 65℃ for 2 to 8 hours, then pour of prehybridization solution; it can be stored at 4C and reused over a week or so.Hybridization solution FinaldH2O50% dextran sulfate 1M Tris pH 8.0 0.5M EDTA pH 8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml
deg.C). Kanamycin sulfate (Kan): Stock of 5 mg/ml in sterile double distilled water (sddH2 O). 0.5 g Kanamycin (Boehringer Mannheim 106 801) sddH2 O to 100 ml (Add to media for final conc. 20 ug/ml) 1M KCl (potassium chloride): 7.5 g KCl ddH
In Situ HYBRIDIZATION TO TISSUE SECTIONS
(do under the hood): -Place racked slides in 500 ml 0.1M Triethanolamine, pH 8.0 (fresh; titrate with HCl). -While stirring rapidly, add 1.25 ml acetic anhydride drop by drop to ensure proper mixing. Incubate 10' during which time the stirring may be slowed
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