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货号:T_701OB6170-01
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英文名称:RABBIT ANTI MOUSE IG UNL 1.0MG
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文献和实验Wholemount staining of embryos
as above 15 to 30''. Secondary antibody is added in blocking solution. Concentration varies, but 1/500 works for most. For monoclonal primaries use anti-mouse coupled to AP or HRP, for polyclonals use anti-rabbit coupled AP or HRP. Incubate at 4 degC
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
3% hydrogen peroxide (H 2 O 2 ) diluted in water (Sigma‐Aldrich) M.O.M. mouse Ig blocking reagent (Vector Laboratories) 1×
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
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