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文献和实验, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Methods for Meta‐Analysis of Genetic Data
, using genome?wide genotype data, are often underpowered. Meta?analyses of multiple studies performing genome?wide genotyping improve power and have led to the identification of thousands of genotype?trait associations. This unit provides an overview
that soaking worms in dsRNA was also able to induce silencing (14). These strategies, whereby large numbers of nematodes are exposed to dsRNA, have enabled large-scale screens to select for RNAi-defective C. elegans mutants and have led to large numbers of gene
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