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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Procedures for In Vitro Production of Bovine Embryos
to hold the ovary firmly in place. The excess tissue from the ovarian stalk is removed in panels B. Panels B and C shows how the ovary is held above the beaker and 2 � 3 mm incisions made in a a downward direction with a rapid but firm movement
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