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- T_7015839-16
- 2025年07月12日
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货号：T_7015839-16
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英文名称：PHOSPHATE STD, 1000 PPM, 500ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
Lacrimal Experimental Protocol (4/4/94)
enzyme solution mixing together 500 µl of hyaluronidase (Worthington 'HSEP'; stock 6980 U/ml), 500 µl of collagenase (Worthington; 1:1 mixture of types II and IV; stock 4000 U/ml), and 100 µl of DNase (stock 1000 U/ml) to 8.4 ml of DMEM/gent.
Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
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