赛默飞世尔Thermo Fisher pH electrod

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

美国实验室wetern方法

and store at -20°C. 2x Sample Buffer (Hoeffer Scientific) (125 mM Tris-HCl, 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromophenol blue). Prepare in fume hood! 2.5 ml 0.5 M Tris-HCl, pH6.8 and 4.0 ml of 10% SDS (--or-- 2.5 ml of upper gel

Pulse Field Electrophoresis

useful portion of the gel compared to FIGE. Figure 1: Electrode configuration of commonly used pulsed field gel electrophpresis units