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文献和实验Construction of homemade 'T-vectors'
This method is after Marchuk,D.,et al.,1991,Nucl.Acids Res.19(5),pp1154. You will need: 10 x Taq buffer (Promega) Taq Polymerase (Promega) Phenol/chloroform mix 100mM dTTP TE buffer Absolute ethanol 70% ethanol
will fill 2 ultracentrifuge tubes);2. Add 5 volumes (v/w) homogenization buffer (4M guanidine thiocyanate, 25mM sodium citrate pH 7.0, 1.5% sarcosyl-0.1 M 2- mercaptoethanol added just prior to using) to ground tissue in a beaker or sterile Falcon tube and homogenize
Construction of homemade T-vectors
Construction of homemade 'T-vectors' This method is after Marchuk, D., et al., 1991, Nucl. Acids Res. 19(5), pp 1154. You will need: 10 x Taq buffer (Promega) Taq Polymerase (Promega) Phenol/chloroform mix 100mM dTTP TE
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