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999
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文献和实验RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T
Preparation and Analysis of Glycan Microarrays
., Smith, D.F., Wilce, M.C., Talbot, U.M., Chong, D.C., Yu, H., Huang, S., Chen, X., Varki, N.M., Varki, A., Rossjohn, J., and Beddoe, T. 2008. Incorporation of a non‐human glycan mediates human susceptibility to a bacterial toxin. Nature 456:648‐652
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