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999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_7019200-16
中文名称：
英文名称：WATER HARDNESS BUFFER, 500 ML
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文献和实验Purification of Plasmid from 50 ml-culture
at room temperature in a plastic bottle. Don''t autoclave. Solution III 5M potassium acetate 60 ml glacial acetic acid 11.5 ml Distilled water 28.5 ml Store at room temperature. This solution can be autoclaved. However, we usually
CCMB buffer to make chemical competent cell
. 3. Preparing competent cells Inoculate 250 ml of SOB medium with 1 ml vial of seed stock and grow at 20 C to an OD of 0.3 This takes approximately 16 hours. Controlling the temperature makes this a more reproducible process, but is not essential. Room
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
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