赛默飞世尔Thermo Fisher METHYLENE B

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

of 70‐ to 250‐ml volume (e.g., CBS Scientific GM‐150 or GM‐200 or Hoefer SG500) Magnetic stirrers and stir bars Graduated cylinder Adhesive tape 1.5‐mm Teflon combs (10‐, 15‐, or 20‐tooth

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

重组DNA的分离、克隆与测序实验手册

for ease in reaction pipetting. One 40 ml preparation of buffer will be sufficient for one batch (about 200 tubes) of A, C, G, and T mix aliquots. 2. Taq Dilution Buffer 400 mM Tris-HCl, pH 9.0 16 ml 1 M Tris-HCl, pH 9.0 100 mM (NH4)2SO4, pH