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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311817843
中文名称：
英文名称：Comb for Verti-gel maxi gels PAGE 5-sample
货期：热销品现货，其他请咨询
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文献和实验Preparation of PAGE gels.1. Clean glass plates with ethanol and assemble casting stand, see Biorad instruction manual2. Mix solutions for lower gel in order shown ie. TEMED last and pour into plates leaving about 2cm
for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially
Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE)
until the top is reached. Carefully place an appropriate comb into each gel, taking care not to trap any bubbles. Allow gel to polymerize for about 1 hour. Remove the front faceplate of the caster. Carefully remove the gels
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