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文献和实验Purification of Plasmid from 50 ml-culture
at room temperature in a plastic bottle. Don''t autoclave. Solution III 5M potassium acetate 60 ml glacial acetic acid 11.5 ml Distilled water 28.5 ml Store at room temperature. This solution can be autoclaved. However, we usually
Purification of Plasmid from 50 ml-culture
of the tube with 500 ul of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
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