赛默飞世尔Thermo Fisher SULFURIC AC

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

v468 Chapter 2 Wnt活性蛋白分离及应用

a change in specific activity. 3.2. Preparation of Wnt3A CM for Fractionation 1. To the filtered CM from Section 3.1 , add Triton X-100 to1% (v/v), Tris-HCl, pH 7.5, to 20 mM, and NaN 3 to 0.01%(w/v) (e.g., to 930 mL CM, add 50 mL of 20% (v/v) TritonX

V294.PART II，Chapter 2 Boyden法体外趋化试验

9791; Sigma-Aldrich): dissolve collagen at 1mg/mL in 0.1 N acetic acid. Allow to stir at room temperature until dissolved(takes 1–3 h). Keep collagen stock at 4°C. 9. Methanol. 10. Giemsa stain, modified solution (cat. no. GS500; Sigma-Aldrich