赛默飞世尔Thermo Fisher HYDROCHLORI

常用缓冲液配制

NaCl Conc. Stock Volume 10 mM 1 M Tris pH 7.5 100 µl 15 mM 5 M NaCl 30 µl H2O to 10 ml Store at 4 C. 10X TBE Buffer (pH 8) 1X 1L 10X 4L 10X 89 mM Tris-Base 108 g 432 g 89 mM Boric acid 55 g 220 g 2 mM Na2EDTA 9.3 g 37.2 g

Working Procedures in Microwave Histology

% ethanol, two changes, 1 minute each 70% ethanol, 1 minute Rinse in distilled water, 1 minute Citrate buffer: 10mM Citrate Buffer (2.1g Citric Acid to 1L of distilled water, adjust pH to 6.0 with approximately 10mL of 2M NaOH

单组分溶液配制

, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M