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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
while imaging. Evaluate the axon after triggering the laser and slowly increase laser power until the axon is cut. You will need about 10X more laser power to cut axons than to form an ablation spot on the alignment target slide (about 1uJ/ ns pulse
Preparation of low density, collagenase-digested splenocytes Protocol
fragments behind, transfer solution to tube. 4. Add 10 ml 400U/ml collagenase to dish with leftover fragments and incubate at 37oC for 60-90 minutes. Then pipette fragments vigorously and mush with glass slides. Rinse slides with 3 ml 100U/ml collagenase
Beta-galactosidase Reporter Gene Assay (Liquid Form)
Beta-galactosidase Reporter Gene Assay (Liquid Form) REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
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