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货号：T_701F171502G
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Procedures for In Vitro Production of Bovine Embryos
)Disposable scalpels #21 (08-927-5D) Dolphin-nose tip microcentrifuge tubes, 1.5 ml (07200210) (Costar 3213) Drummond microdispenser (2117010) (Drummond Sci 3-000-105) Drummond microdispenser replacement tips (Drummond Sci 3-000-105-G) (Fisher
Gene Knockout Techniques(1) The Knockout Process
of pelleted worms for each set of five or six plates. (You?ll discard some worms, but five to 10 150mm plates is LOTS of mutagenized worms. Don?t sweat it.) Put the plates in the dark at 20oC to 22o overnight to recover.Synchronize and set up to screen
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