赛默飞世尔Thermo Fisher 蛋白低吸附管 X100
Test tube Eppendorf protein LoBind 1.5mL (pack of 100) 货号：T_70310708704- ￥70 - 1400
- Thermo Fisher
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- T_70310708704
- 2025年07月13日
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- 库存：
999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70310708704
中文名称：蛋白低吸附管
英文名称：X100 Test tube Eppendorf protein LoBind 1.5mL (pack of 100)
货期：热销品现货，其他请咨询
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文献和实验. 5. Transfer a 400-μL aliquot of the cell suspension into a sterile1.5-mL Eppendorf tube. 6. Add 30 μg of plasmid DNA into the 1.5-mL Eppendorftube. Gently mix the cells and DNA by pipetting the solutionup and down. Keep mixture for 10 min at RT
For 500,000 cells, lyse with 20 µl. Spin at 14,000 rpm (16,000 g) in an Eppendorf microfuge for 10 min at 4°C. Transfer the supernatant to a new tube and discard the pellet. Determine the protein concentration (Bradford assay, A280
0.5-1ml 2) Incubate on ice for 10min. Scrape into microfuge tube, vortex for 10”, then ice for an additional 10min. 3) Spin at 4OC for 10min at 14000rpm (Eppendorf microfuge; ~16000xg) 4) Transfer supernatant lysate to new, pre-chilled microfuge
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